You are examining fibroblast c
You are examining fibroblast cells in the laboratory, and decideto alter their conditions and test the affect on cell migration.What effect would you expect if:
a) You coated the slide with a single stripe of fibronectin?
b) Treated them with a Rho kinase inhibitor?
c) Treated them with a drug that activated WASP and Arp2/3?
Answer:
1. Answer:
When fibroblast cells are coatedwith fibronectin stripes, the cell elongates itself along the majoraxis of the pattern. The nucleus of the cell elongated due to thecompressive pressure applied by cytoskeletal microtubule motorswhich was applied by actin stress fibers. Hence, the extent ofnucleus elongation can exhibit multimodal behaviour among some celllines such as human mesenchymal stem cells (HMSCS), so that thehistogram of aspect ratio of the HMSCS shows two distinct groups ofelongated and round nucleus. Because of the pivotal role thatnuclear deformation plays in the behaviour and fate of cells,substantial interest has developed exploring this opportunity tocontrol the deformation using substrate topography particularly forcontrol of proliferation and differentiation of stem cells.
2. Answer:
when treated with Rho-associatedprotein kinase (ROCK) inhibitors reduces migration and poliferationof fibroblast cells. for example, Prevention of transforming growthfactor β (tgfβ)-induced transdifferentiation of cultured scleralfibroblasts to myofibroblasts by Rho-associated protein kinase(ROCK) inhibitors. Additionally, they tested whether local deliveryof ROCK inhibitors reduced scleral fibroblast proliferation inresponse to chronic intraocular pressure (IOP) elevation. Thesereduce tgfβ-induced myofibroblast transdifferentiation andglaucoma-induced scleral cell proliferation.
3. Answer:
The Arp2/3 complex – an actinfilament nucleating and organizing factor – appears to be a centralplayer in the cellular control of actin assembly. Recently, amolecular pathway leading from key signalling molecules to actinfilament nucleation by the Arp2/3 complex has been discovered. Inthis pathway, the GTPase CDC42 acts in concert with WASP familyproteins to activate the Arp2/3 complex. The coordination of cellshape change and locomotion requires that actin polymerization atthe cell cortex be tightly controlled in response to bothintracellular and extracellular.
Note: The provided answer is as per my knowledge may be or maynot be 100% correct, but definitely relevant to your question thankyou so much.
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