Two ionization methods – elect

Two ionization methods – electron ionization and chemicalionization. Briefly discuss the process involved in ion formationfor each method, and discuss the advantages/disadvantages of eachmethod in identification of an unknown compound.


Electron ionization (EI,formerly known as electron impactionization[1] and electron bombardmentionization[2]) is an ionization method in whichenergetic electrons interact with solid or gas phase atoms ormolecules to produce ions.[3] EI was one of the firstionization techniques developed for massspectrometry.[4] However, this method is still a popularionization technique. This technique is considered a hard (highfragmentation) ionization method, since it uses high energeticelectrons to produce ions. This leads to extensive fragmentation,which can be helpful for structure determination of unknowncompounds.

Advantages Disadvantages
Simple Molecule must be volatile
Sensitive molecule must be thermally stable
Fragmentation aids Identification of molecules Extensive fragmentation- can’t interpret data

Library-searchable fingerprint spectracalionization (CI) is an ionizationtechnique used in mass spectrometry.

Chemical ionization (CI) is anionization technique used in mass spectrometry.Chemical ionization is a lower energy process thanelectron ionization (EI). The lower energy yieldsless or sometimes no fragmentation, and usually a simplerspectrum.

Chemical IonizationAdvantages: Milder ionization than EI ;reduces fragmentation. Requires lower energy than EI. Often givesmolecular weight information through molecular-like ions such as[M+H]+, even when EI would not produce a molecular ion.

Disadvantage :- no fragmentlibrary

Need volatile samples,need thermalstability,picimolar samples, low mass compound(>1000DA)

Useful mass range is low (<1000 Da)

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