TSA medium with 5% sheep blood

TSA medium with 5% sheep blood cells (RBCs) is used fordetermining the hemolytic capabilities ofan organism. Some bacteria produce exoenzymes that lyse red bloodcells and degrade hemoglobin;these are called hemolysins. Bacterial hemolysis includes.a. Beta-hemolysin breaks down the RBCs and hemoglobin completely[β-hemolysis]b. Alpha-hemolysin partially breaks down the RBCs and leaves agreenish color behindc. No production of hemolysins equals no breakdown of RBCs and iscalled γ-hemolysis.d. a and be. all the above

22. When asked to identify several unknown bacteria isolatedfrom the WCCCD restroom, laboratoryanalysts used microscopic, macroscopic, and biochemical tests inorder to characterize theunknowns. In order to determine the genus of their unknowns, itwould be making most sense if theanalyst utilized which of the following documents.a. Foundations in Microbiology, Tenth Edition, Talaro,McGraw-Hillb. Benson’s Microbiological Applications, 13 th Edition, Brown,Alfred Ec. Bergey’s Manuald. Techniques for Studying Bacteria and Fungi Manual23. A rod-shaped, Gram-positive, spore forming with a chainarrangement is most likely to bea. Staphylococcus aureusb. Escherichia colic. Bacillus megateriumd. Mycobacterium smegmatise. Micrococcus luteus24. A rod-shaped, Gram-negative, bacteria that produces a greenpigment is most likely to bea. Escherichia colib. Micrococcus luteusc. Proteus vulgarisd. Pseudomonas aeruginosae. Euglena gracilis


Question 1:-

The right answer is option (e) All of the above

Explanation :- Hemolysis test is used to determine the presenceof an enzyme called Hemolysin and this is of two types. The enzymewhich break down cell of our blood is known as Beta Hemolysin andis produced by species like Staphylococcus aureus. On the otherhand, Alpha Hemolysin do not break down red blood cells completelyand is characteristic of bacteria like Streptococcuspneumoniae. Some bacteria are unable to lyse the red bloodcells and are called gamma Hemolysin.

Question 22 :-

The right option is option (C) Bergey’s manual

Explanation :- Bergey’s manual was first published by DavidBergey and it is the go to book for any researcher if they want toidentify a prokaryotic organism. Whatever aspect you want to use toidentify a given sample, Bergey’s manual cover it and using thatyou can find out the given species. On the other hand, all theother books are Microbiology Textbook or labbooks while Bergey is amanual for identification.

Question 23 :-

The right option is option (c) Bacillus megaterium

Explanation :- Bacillus and Clostridium are the two genus ofbacteria which are Gram positive rods and capable of formingspores.

On the other hand, Escherichia coli is Gram negative and nonspore forming, Staphylococcus aureus is a coccus not rodshaped.

Mycobacterial species aren’t stained by Gram stain whileMicrococcus is also a coccus not a rod shaped bacteria.

Question 24:-

The right option is option (d) Pseudomonas aeruginosa

Explanation :- P. aeruginosa is capable of producing pigmentslike pyocyanin which make it appear greenish in cultures. Whileother options available aren’t capable of producing greenpigments.

Meanwhile Euglena isn’t a bacterium but a protists which areconsidered eukaryotes.

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