Distinguish between the steril

Distinguish between the sterilization of microbiological mediaand the pasteurization of dairy products.

Why is ionizing radiation more effective than UV radiation forsterilization of food products?

Distinguish between a sterilant, a disinfectant, and anantiseptic. What is cold sterilization?


1).Distinguish between the sterilization of microbiologicalmedia and the pasteurization of dairy products

Answer-  Microbiological media sterilization happensin an Autoclave. a Autoclave is widespread heating device. it usessteam under pressure for a certain time period.For heat sensitive products like dairy, pasteurization in used….Pasteurization is applied for the treatment of dairy products. thatinclude thermolabile ingredients such as sugars and proteins, as afood preservation method in the food industry, etc. Because itkills only vegetative forms on the other hand sterilisation killsall forms of pathogens including spores so used mainly for surgicalinstruments and inanimate objects.  

You would not want to sterile the milk because then it willdestroy all the good nutrients, vitamins and flavor. So bypasteurisation we only increase the shelf life of milk bydestroying vegetative forms of bacteria.

2).Why is ionizing radiation more effective than UV radiationfor sterilization of food products?

Answer,-. ionizing radiation is electromagnetic. when this typeradiation hits they release reactive molecules like OH, e-, and H.the reactive molecules tear down lipids, proteins and DNA untilcell death.UV radiation is difference in wave length which leads to DNAmodifaction or breakdown.Ionizing is more effective because it penetrates deeper…..

Also ultraviolet can only be used to kill bacteria onsurfaces

3).Distinguish between a sterilant, a disinfectant, and anantiseptic. What is cold sterilization?….

Sterliant- Sterilant – a chemical agent that destroys all formsof microbial life, including spores. Ethylene oxide is an exampleof a sterilant used to sterilize medical equipment at a lowtemperature.

Disinfectant- usually a chemical agent but sometimes a physicalagent that destroys disease causing pathogens or other harmfulmicroorganisms but might not kill bacterial spores… It refers tosubsatnces applied to inanimate objects An example of adisinfectant is hydrogen peroxide commonly used as a medicaldisinfectant.

Antiseptic – a chemical agent that kills or inhibits the growthof microorganisms but is sufficiently non-toxic to be applied toliving tissues. Rubbing alcohol is an example of an antisepticagent…


Cold (chemical) sterilization is the use of liquid chemicalsolutions to sterilize medical instruments. Not all items can besterilized using liquid chemicals. To be effective, this methodmust be used on items that are relatively smooth, resistant tomoisture, and exposed on all sides. This means the item that isplaced inside the chemical sterilant must have a shape where theliquid sterilant can contact all of its surfaces at once. If acomplex instrument is being placed into the solution it should befully opened up or disassembled prior to sterilization.

Additionally, items placed in the cold sterilant must be cleanand dry. This means all tissue, blood, pus, saliva, and the likehave to be physically cleaned off of the item prior to being placedinto the solution. All of that gory stuff is a really great way toinsulate microorganisms from the sterilant. They are basically likeshields around microorganisms because the chemical sterilant has atough time penetrating through them.

Cold sterilization is a method of sterilization that requiresthe reusable semi-critical items to be immersed in EPA-approvedliquid chemicals. These chemicals can include glutaraldehydes,peracetic acid, and hydrogen peroxide-based solutions. Single-useitems can never be cold sterilized. They are not manufactured to besterilized and may resist the process because of the materials withwhich they are made.

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