1. These enzymes first discove

1. These enzymes first discovered in bacteria, can cut within aDNA strand at specific palindromic consensus sequences, producingmultiple fragments with blunt or “sticky” ends

peptidyl transferases




2. After binding of mRNA, the next step in translationinitiation is binding of formyl-methionine-tRNA

in the P site of the large ribosomal subunit

in the E site of the small ribosomal subunit

in the P site of the Small ribosomal subunit

In the A site of the large ribosomal subunit

in the A site of the small ribosomal subunit

in the P site of the larger ribosomal subunit

3. Eukaryotic pre-mRNA transcripts undergo extensive processingin the nucleus to produce mature mRNA for transport to ribosomes inthe cytoplasm. Describe the 3 most important steps in thisprocess.



1). exonucleases


Exonuclease firstattacks on the phosphodiester bond which removes the nucleotidesone by one from the polynucleotide chain. The ends which areoverhanging from the strand refers to “Cohesive or sticky ends”

2). in the P site of the largeribosomal subunit

Explanation: The first amino acid isformyl-methionine is the first amino acid in protein synthesis inprokaryotes. The first amino acid occupies on the P site of largeribosomal unit.

3). Pre.mRNA undergoes modificationbefore translation. These are considered as post-transcriptionalmodifications.

  1. 5’ Capping: The 5’capping reaction replaces the triphosphate group at the 5′ end ofthe RNA chain with a special nucleotide that is referred to as the5′ cap. It is thought to help with mRNA recognition by the ribosomeduring translation.
  2. Polyadenylation: Amodification also takes place at the opposite end of the mRNAtranscript. To the 3′ end of the RNA chain 30-500 adenines areadded in what is called a poly A tail.
  3. Splicing: Removalof introns is called splicing.
  4. Alternatesplicing: Alternative pattern of RNA splicing is adaptedfor the synthesis of tissue-specific proteins.

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